A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques

• Published in: Forensic science international Vol. 53; no. 1; pp. 29 - 43
• Main Authors: Gill, P., Woodroffe, S., Bär, W., Brinkmann, B., Carracedo, A., Eriksen, B., Jones, S., Kloosterman, A.D., Ludes, B., Mevag, B., Pascali, V.L., Rudler, M., Schmitter, H., Schneider, P.M., Thomson, J.A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ireland Ltd 1992; Elsevier
• Subjects: Autoradiography; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; DNA - blood; DNA Fingerprinting - standards; EDNAP; Electrophoresis, Agar Gel; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Interlaboratory comparisons; Laboratories - standards; Quality Control; Restriction Mapping; Single locus probes; Sizing; Europe; EDNAP; Interlaboratory comparisons; Sizing; Single locus probes; Human; Restriction fragment length polymorphism; Multicenter study; Chromosome DNA; Genetic identification; Forensic aspect; Method; Experimental protocol
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/0379-0738(92)90130-O

This paper describes a collaborative exercise intended to demonstrate whether uniformity of DNA profile results could be achieved between different European laboratories. It was shown that this goal can be obtained provided that a common protocol is followed (specifically the use of a common electrophoretic buffer as being the most important parameter). Generally, lower molecular weight loci (with lower molecular weight fragments) such as YNH24 perform better than higher molecular weight loci such as MS43a. The results of the exercise are discussed in relation to the objectives of the European DNA profiling group (EDNAP).