Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

• Published in: The plant pathology journal Vol. 35; no. 5; pp. 538 - 542
• Main Authors: Choi, Go-Woon, Oh, June-Pyo, Cho, In-Sook, Ju, Hye-Kyoung, Hu, Wen-Xing, Kim, Boram, Seo, Eun-Young, Park, Jong-Seok, Domier, Leslie L, Hammond, John, Song, Kihak, Lim, Hyoun-Sub
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.10.2019; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: difference of infectivity; full-length infectious cDNA clone; tobamovirus; tomato mosaic virus (ToMV); 농학; difference of infectivity; full-length infectious cDNA clone; tomato mosaic virus(ToMV); tobamovirus
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.NT.12.2018.0286

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.