Acetylcholine in the crayfish optic lobe: concentration profile and cellular localization

The crayfish optic lobe contains high levels of acetylcholine (ACh) and choline as measured with a chemiluminescent assay in small fragments of optic lobe tissue. The highest concentrations were found in the medulla externa and medulla interna (second and third optic neuromeres), which have ACh conc...

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Bibliographic Details
Published inThe Journal of neuroscience Vol. 9; no. 6; pp. 1864 - 1871
Main Authors Wang-Bennett, LT, Pfeiffer, C, Arnold, J, Glantz, RM
Format Journal Article
LanguageEnglish
Published Washington, DC Soc Neuroscience 01.06.1989
Society for Neuroscience
Subjects
Acetylcholine - metabolism
Animals
Astacoidea - metabolism
Biochemistry. Physiology. Immunology
Biological and medical sciences
Choline - metabolism
Crustacea
Freshwater
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Invertebrates
Optic Lobe, Nonmammalian - cytology
Optic Lobe, Nonmammalian - metabolism
Osmolar Concentration
Physiology. Development
Procambarus clarkii
Tissue Distribution
Immunohistochemistry
Macrura
Optic lobe
Crustacea
Freshwater environment
Optical microscopy
Visual pathway
Synaptic transmission
Arthropoda
Neuromediator
Acetylcholine
Decapoda
Procambarus clarkii
Invertebrata
Localization
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Summary:The crayfish optic lobe contains high levels of acetylcholine (ACh) and choline as measured with a chemiluminescent assay in small fragments of optic lobe tissue. The highest concentrations were found in the medulla externa and medulla interna (second and third optic neuromeres), which have ACh concentrations of 270 pmol/mg tissue. This concentration is about 16 times that measured in the photoreceptors and lamina ganglionaris (the first optic neuromere). Immunocytochemistry (based upon antisera to choline-glutaryl-BSA) revealed low levels of ACh-like reactivity in the lamina ganglionaris associated with the terminal arbors of centrifugal and/or tangential neurons. The most intense ACh-like reactivity was observed in monopolar neurons of the medulla externa and medulla interna. One monopolar neuron/medullary column (or about 2500 neurons/medullary neuropile) exhibited reactivity and an estimated cytoplasmic concentration of 8.1 mM.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.09-06-01864.1989