A host-vector system for a cellulose-producing Acetobacter strain

• Published in: Bioscience, biotechnology, and biochemistry Vol. 58; no. 10; pp. 1899 - 1901
• Main Authors: Tonouchi, Naoto, Tsuchida, Takayasu, Yoshinaga, Fumihiro, Horinouchi, Sueharu, Beppu, Teruhiko
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1994; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: acetobacter; Acetobacter - genetics; Acetobacter - metabolism; Amino Acid Sequence; Ampicillin Resistance - genetics; Base Sequence; Biological and medical sciences; Biotechnology; cellulose; Cellulose - biosynthesis; Cellulose - genetics; celluloses; celulosa; DNA, Bacterial - biosynthesis; Escherichia coli - metabolism; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Genetic Vectors; genie genetique; ingenieria genetica; Methods. Procedures. Technologies; Molecular Sequence Data; Open Reading Frames; plasmide; plasmidios; Plasmids; Sequence Homology, Nucleic Acid; Vectors (cloning, transfer, expression). Insertion sequences and transposons; Acetobacteraceae; Plasmid; Genetic transformation; Nucleotide sequence; Escherichia coli; Shuttle vector; Bacteria; Electroporation; Enterobacteriaceae; Acetobacter
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.58.1899

An indigenous plasmid, named pAH4, was detected in a cellulose-producing Acetobacter strain. This plasmid, consisting of 4002 bp, contained an AT-rich region and encoded several open reading frames, as deduced by the complete nucleotide sequence. One of the putative open reading frames showed homology with replication proteins of other plasmids. A shuttle vector of Escherichia coli and this strain was constructed by connecting pAH4 to pUC18. Electroporation of the shuttle vector into the strain yielded 1.7 × 10 5 ampicillin resistant transformants per μg DNA. The shuttle plasmid was very stably maintained in the strain.