Expression of surrogate light chain receptors is restricted to a late stage in pre-B cell differentiation

• Published in: Cell Vol. 73; no. 1; pp. 73 - 86
• Main Authors: Lassoued, K., Nuñez, C.A., Billips, L., Kubagawa, H., Monteiro, R.C., LeBien, T.W., Cooper, M.D.
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 09.04.1993; Cell Press
• Subjects: Animals; Antibodies, Monoclonal; Antigens, Surface - metabolism; B-Lymphocytes - cytology; Biological and medical sciences; Cell Differentiation - immunology; Cell Line; Epitopes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Hybridomas; Immunobiology; Immunoglobulin Light Chains - metabolism; Immunoglobulin Light Chains, Surrogate; Immunoglobulin mu-Chains - metabolism; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Membrane Glycoproteins - metabolism; Mice; Models, Biological; Phenotype; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - immunology; Pseudogenes; Receptors, Immunologic - metabolism; Tumor Cells, Cultured; Cytometry; Immunoglobulins; Membrane receptor; Investigation method; light-Peptide chain; B-Lymphocyte; Monoclonal antibody; Cell differentiation
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/0092-8674(93)90161-I

Surrogate light chain (ψLC) genes are transcriptionally active in progenitor B (pro-B) cells before immunoglobulin genes are rearranged. Current hypothetical models suggest that the ψLC proteins may couple with surrogate or conventional heavy chain proteins to form cell surface receptors that signal the progressive differentiation of pro-B, precursor B (pre-B), and immature B cells. Monocional antibodies were produced and used to examine the synthesis, expression, intermolecular interaction, and function of ψLC during B cell differentiation. The results indicate that, while ψLC production spans several developmental stages, cell surface expression is confined to a relatively late stage in normal pre-B cell differentiation, during which receptor cross-linkage does not impede cell growth or B cell differentiation.