5-ALA fluorescence in randomly selected pediatric brain tumors assessed by spectroscopy and surgical microscope

• Published in: Acta neurochirurgica Vol. 165; no. 1; pp. 71 - 81
• Main Authors: Milos, Peter, Haj-Hosseini, Neda, Hillman, Jan, Wårdell, Karin
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.01.2023; Springer Nature B.V
• Subjects: 5-ALA; Adolescent; Aminolevulinic Acid; Brain cancer; Brain Neoplasms - diagnostic imaging; Brain Neoplasms - pathology; Brain Neoplasms - surgery; brain tumor; Brain tumors; Cerebellar Neoplasms; Child; Child, Preschool; Children; Fluorescence; Glioblastoma; Glioma; Glioma - diagnostic imaging; Glioma - pathology; Glioma - surgery; Humans; Interventional Radiology; Medicine; Medicine & Public Health; Meningioma; Minimally Invasive Surgery; Neurology; Neuroradiology; Neurosurgery; Original - Pediatric Neurosurgery; Original Article - Pediatric Neurosurgery; Patients; Pediatrics; Photosensitizing Agents; Side effects; Skin; Spectroscopy; Spectrum Analysis - methods; Surgery; Surgical Orthopedics; Tumors; Brain tumor; Spectroscopy; Children; 5-ALA
• ISSN: 0942-0940; 0001-6268; 0942-0940
• DOI: 10.1007/s00701-022-05360-1

Purpose Fluorescence-guided surgery applying 5-aminolevulinic acid (5-ALA) in high-grade gliomas is an established method in adults. In children, results have so far been ambiguous. The aim of this study was to investigate 5-ALA-induced fluorescence in pediatric brain tumors by using the surgical microscope and a spectroscopic hand-held probe. Methods Fourteen randomly selected children (age 4–17) with newly MRI-verified brain tumors were included. No selection was based on the suspected diagnosis prior to surgery. All patients received 5-ALA (20 mg /kg) either orally or via a gastric tube prior to surgery. Intratumoral fluorescence was detected with the microscope and the probe. Moreover, fluorescence in the skin of the forearm was measured. Histopathology samples revealed seven low-grade gliomas, four medulloblastomas, one diffuse intrinsic pontine glioma, one glioblastoma and one atypical meningioma. Blood samples were analyzed, and potential clinical side effects were monitored. Results Microscopically, vague fluorescence was visible in two patients. Intratumoral fluorescence could be detected in five patients with the probe, including the two patients with vague microscopic fluorescence. Three of the oldest children had PpIX fluorescence in the skin. Nine children did not show any fluorescence in the tumor or in the skin. No clinical side effects or laboratory adverse events were observed. Conclusion Fluorescence could not be used to guide surgery in this study, neither with the surgical microscope nor with the hand-held probe. In nine children, no fluorescence was discerned and children with noticeable fluorescence were all older than nine years. 5-ALA was considered safe to apply in children.