Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method

Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit approximately 25–40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current standard method of DENV serot...

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Published inSensors (Basel, Switzerland) Vol. 21; no. 23; p. 7809
Main Authors Poltep, Kanaporn, Nakayama, Emi E., Sasaki, Tadahiro, Kurosu, Takeshi, Takashima, Yoshiki, Phadungsombat, Juthamas, Kosoltanapiwat, Nathamon, Hanboonkunupakarn, Borimas, Suwanpakdee, Sarin, Imad, Hisham A., Srimark, Narinee, Kitamura, Chiaki, Yamanaka, Atsushi, Okubo, Akio, Shioda, Tatsuo, Leaungwutiwong, Pornsawan
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 24.11.2021
MDPI
Subjects
Antibodies
Dengue fever
dengue virus
Disease
Epidemics
Ethics
immunochromatography
Infections
Laboratory animals
Mortality
NS1
rapid test
serotype-discrimination
Viruses
Brazil
Thailand
Japan
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Summary:Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit approximately 25–40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current standard method of DENV serotype determination. However, since this method is time-consuming, rapid detection systems are desirable. We established several mouse monoclonal antibodies directed against DENV non-structural protein 1 and integrated them into rapid DENV detection systems. We successfully developed serotype-specific immunochromatography systems for all four DENV serotypes. Each system can detect 104 copies/mL in 15 min using laboratory and clinical isolates of DENV. No cross-reaction between DENV serotypes was observed in these DENV isolates. We also confirmed that there was no cross-reaction with chikungunya, Japanese encephalitis, Sindbis, and Zika viruses. Evaluation of these systems using serum from DENV-infected individuals indicated a serotype specificity of almost 100%. These assay systems could accelerate both DENV infection diagnosis and epidemiologic studies in DENV-endemic areas.
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Kanaporn Poltep and Emi E. Nakayama contributed equally to this work.
ISSN:1424-8220
1424-8220
DOI:10.3390/s21237809