new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes

• Published in: Molecular plant-microbe interactions Vol. 17; no. 8; pp. 846 - 852
• Main Authors: Tytgat, T, Vanholme, B, Meutter, J. de, Claeys, M, Couvreur, M, Vanhoutte, I, Gheysen, G, Criekinge, W. van, Borgonie, G, Coomans, A
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.08.2004; American Phytopathological Society; The American Phytopathological Society
• Subjects: Amino Acid Sequence; amino acid sequences; amplified fragment length polymorphism; Animals; Biological and medical sciences; complementary DNA; cultured cells; cyst nematodes; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; gene expression regulation; Globodera; Helminth Proteins - genetics; Helminth Proteins - metabolism; Heterodera schachtii; Host-Parasite Interactions; messenger RNA; Molecular Sequence Data; Nematoda - genetics; Nematoda - pathogenicity; Nicotiana - genetics; Nicotiana - parasitology; Nicotiana tabacum; nucleotide sequences; pharynx; Pharynx - cytology; Pharynx - metabolism; Phytopathology. Animal pests. Plant and forest protection; Plant Roots - parasitology; Random Amplified Polymorphic DNA Technique - methods; signal peptide; tobacco; ubiquitin; Ubiquitin - genetics; Ubiquitin - metabolism; ubiquitin extension proteins; Ubiquitin; Microbiology; Secretion; Helmintha; Nemathelminthia; Invertebrata; Nematoda; Protein
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi.2004.17.8.846

By performing cDNA AFLP on pre- and early parasitic juveniles, we identified genes encoding a novel type of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in the cyst nematode Heterodera schachtii. The proteins consist of three domains, a signal peptide for secretion, a mono-ubiquitin domain, and a short C-terminal positively charged domain. A gfp-fusion of this protein is targeted to the nucleolus in tobacco BY-2 cells. We hypothesize that the C-terminal peptide might have a regulatory function during syncytium formation in plant roots.