Hypoxic stress inhibits multiple aspects of the potato tuber wound response

• Published in: Plant physiology (Bethesda) Vol. 93; no. 1; pp. 264 - 270
• Main Authors: Butler, W. (University of Maine, Orono, ME), Cook, L, Vayda, M.E
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.1990
• Subjects: 550201 - Biochemistry- Tracer Techniques; 560300 - Chemicals Metabolism & Toxicology; ADN; Anaerobic conditions; ANOXIA; ANOXIE; ARN; AZINES; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL STRESS; BIOSINTESIS; BIOSYNTHESE; CODE GENETIQUE; CODIGO GENETICO; Complementary DNA; CURACION; DNA; Fundamental and applied biological sciences. Psychology; GENE; GENE REGULATION; GENES; Genetic hybridization; GUERISON; HERIDAS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; Hypoxia; INHIBITION; INJURIES; ISOTOPE APPLICATIONS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; Metabolism; Molecular Biology and Gene Regulation; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; Photosynthesis, respiration. Anabolism, catabolism; PHYSIOLOGY; PLAIE; Plant physiology and development; PLANTS; Protease inhibitors; PYRIMIDINES; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RIBOSIDES; RNA; RNA probes; SINTESIS DE PROTEINAS; SOLANUM; SOLANUM TUBEROSUM; SYNTHESE PROTEIQUE; THYMIDINE; TRACER TECHNIQUES; TRANSCRIPTION; TRITIUM COMPOUNDS; Tubers; WOUNDS; Solanum tuberosum; DNA synthesis; Gene expression; Wound; Stress; Regulation(control); RNA synthesis; Protein synthesis; Dicotyledones; Angiospermae; Tuber; Hypoxia; Industrial crop; Spermatophyta; Solanaceae
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.93.1.264

Potato (Solanum tuberosum L.) tubers subjected to wounding under hypoxic stress do not synthesize RNA species that are induced in response to wounding in aerobic conditions. Further, wound-response proteins fail to be synthesized when wounded tubers are transferred to hypoxic conditions although messenger RNAs which encode them persist for many hours after transfer. Hypoxic stress also prevents the incorporation of [3H]thymidine by wounded tubers that occurs in aerobic conditions. In contrast, hypoxic tubers accumulate and translate transcripts of genes whose products are involved in anaerobic metabolism whether or not they are wounded. Both the hypoxic response and the aerobic wound response preclude the synthesis of proteins encoded by messenger RNAs which accumulated during the tuberization process and which can be translated in vitro. Finally, wounding elicits the degradation of a subset of these tuberization-associated transcripts. These data indicate a complex and precise regulation of gene expression at several levels of macromolecular synthesis