Zinc-dependent regulation of zinc import and export genes by Zur

• Published in: Nature communications Vol. 8; no. 1; p. 15812
• Main Authors: Choi, Seung-Hwan, Lee, Kang-Lok, Shin, Jung-Ho, Cho, Yoo-Bok, Cha, Sun-Shin, Roe, Jung-Hye
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.06.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 38/1; 38/15; 38/22; 38/39; 38/61; 38/70; 38/88; 45; 45/77; 631/326/325/1506; 631/337/572/2102; 82/80; 82/83; Antibiotics; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding sites; Carrier Proteins - genetics; Carrier Proteins - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression Regulation, Bacterial; Genes; Genomes; Homeostasis; Humanities and Social Sciences; Ion Transport; Metals; multidisciplinary; Promoter Regions, Genetic; Proteins; Science; Science (multidisciplinary); Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Zinc; Zinc - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms15812

In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor , a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression. At the same time, Zur represses genes for zinc uptake. At micromolar zinc concentrations (phase II), oligomeric Zur binding with footprint expansion upward from the Zur box results in high zitB induction. Our findings reveal a mode of zinc-dependent gene activation that uses a single metalloregulator to control genes for both uptake and export over a wide range of zinc concentrations. Zinc homeostasis in most bacteria is achieved by a set of regulators, each responding to a certain level of intracellular zinc. Here the authors show that, in Streptomyces coelicolor , the Zur regulator modulates the expression of genes for zinc import and export over a large range of zinc concentrations.