Optimization of extracellular lipase production by Geotrichum sp. using factorial design

• Published in: Bioresource technology Vol. 91; no. 1; pp. 77 - 84
• Main Authors: Burkert, J.F.M, Maugeri, F, Rodrigues, M.I
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2004; Elsevier Science
• Subjects: ammonium nitrate; Analysis of Variance; Biological and medical sciences; biological production; corn steep liquor; Factorial design; Fundamental and applied biological sciences. Psychology; Geotrichum; Geotrichum - metabolism; Geotrichum sp; Glucose; industrial byproducts; Lipase - biosynthesis; Lipase production; lipases; microbial enzymes; Models, Chemical; Nitrogen; Olive Oil; Optimization; Plant Oils; response surface methodology; Soybean Oil; Surface response analysis; Temperature; Time Factors; Geotrichum sp; Surface response analysis; Factorial design; Lipase production; Optimization; Enzyme; Geotrichum; Triacylglycerol lipase; Esterases; Extracellular; Carboxylic ester hydrolases; Fungi; Hydrolases; Response surface; Fungi Imperfecti; Thallophyta
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(03)00152-4

Response surface methodology was employed to study the effects of carbon source (soy oil, olive oil and glucose) and nitrogen source concentrations (corn steep liquor and NH 4NO 3) on the lipase production by Geotrichum sp. The experiment included a 2 4 central composite rotatable design (CCRD) and four others 2 3 CCRD. According to the responses from the experimental designs, the effects of each variable were calculated and the interactions between them were determined. The response surface methodology was applied for the optimization of the nutrient concentrations in the culture medium for the enzyme production, at 30 °C. The optimum medium composition for lipase production by Geotrichum sp. was ammonium nitrate 2.1–2.5%, corn steep liquor 13–15% and soy oil 0.6% as carbon source, which lead to a lipase activity of about 20 U/ml. Using olive oil as carbon source, the optimum composition was ammonium nitrate 0.8–1%, corn steep liquor 13–15% and olive oil 0.6%, leading to an activity of 17 U/ml.