Insertion of unmarked DNA sequences in multiple loci of the Bacillus subtilis 168 genome: An efficient selection method

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted...

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Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 69; no. 5; pp. 1036 - 1039
Main Authors Uotsu Tomita, R. (Mitsubishi Kagaku Inst. of Life Sciences, Machida, Tokyo (Japan)), Kaneko, S, Tsuge, K, Itaya, M
Format Journal Article
LanguageEnglish
Published Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.2005
Japan Society for Bioscience Biotechnology and Agrochemistry
Subjects
BACILLUS SUBTILIS
Bacillus subtilis - genetics
Bacillus subtilis genome
Biological and medical sciences
Chromosome Mapping
Chromosomes, Bacterial
DNA
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genome, Bacterial
GENOMES
inositol
METHODS
Mutagenesis, Insertional - methods
MUTANTS
NEOMYCIN
pBR322
phleomycin
Recombination, Genetic
SELECTION
TOLERANCE
transformation
Transformation, Bacterial
Inositol
Nucleotide sequence
Bacillus subtilis
Selection
Method
Bacillaceae
transformation
Bacillus subtilis genome
Bacillales
pBR322
Bacteria
phleomycin
Genome
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Summary:The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
Bibliography:U30
2005007896
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.1036