Heterologous expression of C. elegans fat-1 decreases the n-6/n-3 fatty acid ratio and inhibits adipogenesis in 3T3-L1 cells

• Published in: Biochemical and Biophysical Research Communications Vol. 428; no. 3; pp. 405 - 410
• Main Authors: An, Lei, Pang, Yun-Wei, Gao, Hong-Mei, Tao, Li, Miao, Kai, Wu, Zhong-Hong, Tian, Jian-Hui
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.11.2012; Elsevier BV
• Subjects: 3T3-L1 Cells; 60 APPLIED LIFE SCIENCES; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; ADIPOSE TISSUE; Animals; APOPTOSIS; BIOLOGICAL EFFECTS; Caenorhabditis elegans; Caenorhabditis elegans - enzymology; Caenorhabditis elegans Proteins; Caenorhabditis elegans Proteins - biosynthesis; Caenorhabditis elegans Proteins - genetics; CARBOXYLIC ACIDS; Cell Proliferation; DIET; fat-1; FATS; fatty acid composition; Fatty Acid Desaturases; Fatty Acid Desaturases - biosynthesis; Fatty Acid Desaturases - genetics; Fatty Acids, Omega-3; Fatty Acids, Omega-3 - metabolism; Fatty Acids, Omega-6; Fatty Acids, Omega-6 - metabolism; genes; IN VITRO; IN VIVO; LIPIDS; lipogenesis; METABOLIC DISEASES; Mice; obesity; omega-3 fatty acids; Polyunsaturated fatty acids; stearoyl-CoA desaturase; 3T3-L1 cells; Polyunsaturated fatty acids; fat-1; Adipogenesis; Apoptosis

► Expression of C. elegans fat-1 reduces the n-6/n-3 PUFA ratio in 3T3-L1 cells. ► fat-1 inhibits the proliferation and differentiation of 3T3-L1 preadipocytes. ► fat-1 reduces lipid deposition in 3T3-L1 adipocytes. ► The lower n-6/n-3 ratio induces apoptosis in 3T3-L1 adipocytes. In general, a diet...