Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, ar...

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Published inStem cell research Vol. 18; pp. 22 - 25
Main Authors Uhlin, Elias, Rönnholm, Harriet, Day, Kelly, Kele, Malin, Tammimies, Kristiina, Bölte, Sven, Falk, Anna
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.01.2017
Subjects
Cell Line
Cellular Reprogramming
Culture Media - chemistry
Fibroblasts - cytology
Fibroblasts - metabolism
Genetic Vectors - genetics
Genetic Vectors - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Male
Medicin och hälsovetenskap
RNA, Messenger - metabolism
Sendai virus - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Twins, Monozygotic
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Summary:Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2016.12.006