Liquefaction of sugarcane bagasse for enzyme production

• Published in: Bioresource technology Vol. 172; pp. 249 - 252
• Main Authors: Cunha, F.M., Kreke, T., Badino, A.C., Farinas, C.S., Ximenes, E., Ladisch, M.R.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2014; Elsevier
• Subjects: Aspergillus niger; Aspergillus niger - metabolism; Bagasse; Biological and medical sciences; Biotechnology; Cellulase - biosynthesis; Cellulase production; Culture; Enzyme engineering; Enzymes; Fermentation; Food industries; Fundamental and applied biological sciences. Psychology; Liquefaction; Liquefied; Liquids; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Production of selected enzymes; Saccharum; Submerged; Sugarcane bagasse; Use and upgrading of agricultural and food by-products. Biotechnology; Fermentation; Liquefaction; Aspergillus niger; Cellulase production; Sugarcane bagasse; Enzyme; Fungi; Glycosylases; Sugar cane(by product); Cellulolytic enzyme; Cellulase; Glycosidases; Bagasse; Enzyme production; Hydrolases; Fungi Imperfecti
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.09.025

•Submerged fermentation is made possible by liquefaction of biomass.•Liquefaction gives a flowable slurry.•Liquefied biomass is an effective fermentation media.•Liquefied slurry gives 15× higher endoglucanase activity. The objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by Aspergillus niger. After initial solid state fermentation of steam pretreated bagasse solids by A. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30±0.07Pas at 30% (w/v) solids. Addition of A. niger for submerged fermentation of sterile liquefied bagasse at 23% w/v solids resulted in an enzyme titer of 2.5IUmL−1 or about 15× higher productivity than solid-state fermentation of non-liquefied bagasse (final activity of 0.17IUmL−1). Bagasse not treated by initial solid-state fermentation but liquefied with enzyme gave 2IUmL−1. These results show the utility of liquefied bagasse as a culture medium for enzyme production in submerged fermentations.