Molecular biology and application of plant peroxidase genes

• Published in: Applied microbiology and biotechnology Vol. 60; no. 6; pp. 665 - 670
• Main Authors: Yoshida, K, Kaothien, P, Matsui, T, Kawaoka, A, Shinmyo, A
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.02.2003; Springer Nature B.V
• Subjects: Biological and medical sciences; Biosynthesis; Biotechnology; breeding; enzymatic reactions; enzyme immunoassays; Enzyme Induction; Enzymes; Forecasting; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; gene overexpression; Genes; Genes, Plant; Genetic engineering; Genetic technics; Glycoproteins; Horseradish Peroxidase - genetics; Horseradish Peroxidase - metabolism; hybrids; hydrogen peroxide; Hydrogen Peroxide - metabolism; Immunoassays; Isoenzymes - genetics; Isoenzymes - metabolism; isozymes; Lignin; Methods. Procedures. Technologies; Molecular biology; Multigene Family; oxidative stress; oxygen; Oxygen - metabolism; Pathogens; peroxidase; Peroxidases - genetics; Peroxidases - metabolism; Physiology; Plant biology; Plant Development; Plant Proteins - genetics; Plant Proteins - metabolism; Plants - enzymology; Plants - genetics; Plants, Genetically Modified; Potatoes; Promoter Regions, Genetic; salt tolerance; Studies; sweet potatoes; Tobacco; Tomatoes; Transgenic animals and transgenic plants; Transgenic plants; Enzyme; Transcription promoter; Functional analysis; Review; Peroxidases; Enzymatic activity; Gene; Genetic improvement; Peroxidase; Transgenic plant; Oxidoreductases; Molecular biology; Genetically modified organism
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-002-1157-7

Peroxidases are a family of isozymes found in all plants; they are heme-containing monomeric glycoproteins that utilize either H2O2 or O2 to oxidize a wide variety of molecules. These important enzymes are used in enzyme immunoassays, diagnostic assays and industrial enzymatic reactions. Peroxidase genes and their promoters can be used for molecular breeding of useful plants. Transgenic techniques have also been used to investigate the physiological and molecular functions of peroxidase genes in plants. Here, we review transgenic studies of peroxidase genes, including the functional analyses of the enzymes and their promoters. Regarding application of peroxidase genes, it has been reported that overexpression of the tomato TPX2 gene or the sweet potato swpa1 gene conferred increased salt-tolerance or oxidative-stress tolerance, respectively. The growth stimulation effect in transgenic tobacco and hybrid aspen upon overexpression of horseradish peroxidase gene is also discussed.