In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II

• Published in: The plant pathology journal Vol. 35; no. 5; pp. 437 - 444
• Main Authors: Lee, Ye Ji, Jeong, Jin-Ju, Jin, Hyunjung, Kim, Wook, Jeun, Young Chull, Yu, Gyeong-Dan, Kim, Ki Deok
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.10.2019; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: chlorine dioxide; elongation factor 1-α gene; Fusarium oxysporum f. sp. batatas; Fusarium wilt; sweetpotato; 농학; chlorine dioxide; Fusarium oxysporum f. sp. batatas; elongation factor 1-α gene; Fusarium wilt; sweetpotato
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.OA.04.2019.0078

Chlorine dioxide (ClO 2 ) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO 2 gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited upon increasing the concentration and exposure time. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by treatment using various ClO 2 concentrations and treatment times to assess fungus-induced disease development in the slices. Lesion diameters decreased at the tested ClO 2 concentrations over time. When sweetpotato roots were dip-inoculated in spore suspensions prior to treatment with 20 and 40 ppm of ClO 2 for 0–60 min, fungal populations significantly decreased at the tested concentrations for 30–60 min. Taken together, these results showed that ClO 2 gas can effectively inhibit fungal growth and disease development caused by F. oxysporum f. sp. batatas on sweetpotato. Therefore, ClO 2 gas may be used as a sanitizer to control this fungus during postharvest storage of sweetpotato.