Assignment of the Gene for the β Subunit of Thyroid-Stimulating Hormone to the Short Arm of Human Chromosome 1

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 83; no. 6; pp. 1822 - 1826
• Main Authors: Dracopoli, Nicholas C., Rettig, Wolfgang J., Whitfield, G. Kerr, Darlington, Gretchen J., Spengler, Barbara A., Biedler, June L., Old, Lloyd J., Kourides, Ione A.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.03.1986; National Acad Sciences
• Subjects: Animals; Biological and medical sciences; Cattle; Cell Line; Cell lines; Chorionic Gonadotropin - genetics; Chromosome Mapping; Chromosomes; Chromosomes, Human, 1-3; Classical genetics, quantitative genetics, hybrids; Complementary DNA; Cricetinae; Cricetulus; DNA; DNA - genetics; DNA Restriction Enzymes; Fibroblasts; Fundamental and applied biological sciences. Psychology; Genes; Genetic loci; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Glycoproteins; Hormones; Human; Human chromosomes; Humans; Hybrid Cells; Hybridity; L Cells (Cell Line); Melanoma; Mice; Thyrotropin - genetics; Genetic mapping; Human; Pituitary hormone; Gene; TSH; Peptide chain; Glycoprotein hormone; Localization; A1-Chromosome
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.83.6.1822

The chromosomal locations of the genes for the β subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone α subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) α -subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH β -subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG α -subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH β -subunit sequences permitted the assignment of the TSH β -subunit gene to human chromosome 1. The subregional assignment of TSH β subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH β subunit is not syntenic with genes encoding the β subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the β subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS).