Microbial profile of the vitreous aspirates in culture proven exogenous endophthalmitis: A 10-year retrospective study

• Published in: Indian journal of medical microbiology Vol. 34; no. 2; pp. 153 - 158
• Main Authors: Bhattacharjee, H, Bhattacharjee, K, Gogoi, K, Singh, M, Singla, BG, Yadav, A
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.04.2016; Wolters Kluwer - Medknow Publications; Elsevier Limited
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents - therapeutic use; Antibiotics; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Bacterial Infections - epidemiology; Bacterial Infections - microbiology; Cataracts; Child; Child, Preschool; Coinfection - epidemiology; Coinfection - microbiology; Conflicts of interest; Culture positive; Endophthalmitis - epidemiology; Endophthalmitis - microbiology; exogenous endophthalmitis; Female; Fungi; Fungi - classification; Fungi - isolation & purification; Humans; Infant; Male; Microbial Sensitivity Tests; Middle Aged; Mycoses - epidemiology; Mycoses - microbiology; Nosocomial infections; Organisms; outcomes; Pseudomonas; Pseudomonas endophthalmitis; Retrospective Studies; Surgical Procedures, Operative; Treatment Outcome; Young Adult; India; Pseudomonas endophthalmitis; outcomes; Culture positive; exogenous endophthalmitis
• ISSN: 0255-0857; 1998-3646
• DOI: 10.4103/0255-0857.180280

Purpose: To describe the microbiological profile and clinical outcome in the eyes with culture-proven exogenous endophthalmitis. Methods: A retrospective analysis of 495 eyes diagnosed as exogenous endophthalmitis was performed over a period of 10 years. In all, aseptically collected aqueous and vitreous aspirates were cultured for bacteria and fungus using standard microbiological techniques. Gram-stain and KOH preparation of the specimens were also performed. The antibiotic susceptibility testing for bacterial isolates was performed by Kirby–Bauer disk diffusion method. The treatment was modified according to the antibiotic sensitivity profile. The final clinical ocular condition was divided into improved, stable or deteriorated. Results: Of 148 culture-proven endophthalmitis eyes, 137 (92.57%) were referred from elsewhere, and 11 (7.43%) belonged to our institute. Aetiologically, 76 (51.35%) eyes were post-cataract surgery, 61 (41.22%) were post-traumatic, 5 (3.38%) eyes post-intravitreal anti-vascular endothelial growth factor injection, 5 associated with corneal diseases and 1 bleb-related endophthalmitis. In 31 (20.95%) eyes, primary intravitreal antibiotics were given outside. The cultures revealed monomicrobial growth in 92.57% (n = 137) and polymicrobial growth in 7.43% (n = 11). Among the bacteria (n = 121, 81.76%), Pseudomonas species dominated overall (n = 32, 27.11%) and post-operative (n = 26, 38.23%) endophthalmitis group. Staphylococcus epidermidis (n = 14, 28%) was prominent in post-traumatic endophthalmitis group. Ninety-two percent (n = 108 isolates) of bacteria were sensitive to vancomycin. In 78 (52.7%) eyes, the clinical ocular condition improved or remained stable while deteriorated in 51 (34.46%). Conclusion: A bacterial predominance was observed among causative organisms of exogenous endophthalmitis with Pseudomonas species being the most common. The appropriate surgical intervention improved or stabilised the visual acuity in nearly 50% eyes.