Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm r...

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Published inChembiochem : a European journal of chemical biology Vol. 11; no. 10; pp. 1361 - 1363
Main Authors Dellagiacoma, Claudio, Lukinavičius, Gražvydas, Bocchio, Noelia, Banala, Sambashiva, Geissbühler, Stefan, Märki, Iwan, Johnsson, Kai, Lasser, Theo
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 05.07.2010
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
Adenine - analogs & derivatives
Adenine - chemistry
Carbocyanines - chemistry
Cell Line, Tumor
Fluorescent Dyes - chemistry
fluorescent probes
Guanine - chemistry
Humans
Image Processing, Computer-Assisted
Microscopy, Fluorescence
microtubules
Microtubules - ultrastructure
Nanostructures - chemistry
protein structures
SNAP-tag
STORM
Tubulin - chemistry
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Summary:SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.
Bibliography:http://dx.doi.org/10.1002/cbic.201000189
ArticleID:CBIC201000189
Federation of European Biochemical Societies
ark:/67375/WNG-9K7MGTJ2-V
Swiss National Science Foundation - No. CRSII3 125463; K-23K1-116242/1; 315200-116729
istex:4C42AC5E71349B7469BF0B445F222F1BC3E9D254
These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201000189