Microwave-assisted extraction in goji berries: effect on composition and bioactivity, evaluated through conventional and nonconventional methodologies
Summary This study aimed to evaluate the effect of microwave‐assisted extraction (MAE) parameters on the composition and bioactivity of goji (Lycium barbarum) extracts. Extracts were obtained under a central composite design combination of experimental conditions, and characterised through HPLC‐DAD;...
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Published in | International journal of food science & technology Vol. 51; no. 6; pp. 1401 - 1408 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Blackwell Publishing Ltd
01.06.2016
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Summary
This study aimed to evaluate the effect of microwave‐assisted extraction (MAE) parameters on the composition and bioactivity of goji (Lycium barbarum) extracts. Extracts were obtained under a central composite design combination of experimental conditions, and characterised through HPLC‐DAD; their bioactive capacity was ascertained for antimicrobial and antioxidant capacity, the later by spectrophotometric [2,2‐azinobis (3‐ ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt‐radical scavenging activity assay – 413–748 mg ascorbic acid equivalents/100 g DW and oxygen radical absorbance capacity – 1901–2292 mg trolox equivalents/100 g DW] and electrochemical (DNA‐based sensor – 3571–6602 mg ascorbic acid/100 g DW) methods. The quantitative profile of phenolic compounds was strongly dependent on MAE conditions. Significant correlations were found between the presence of several flavonoids and solvent composition, as well as between phenolic acids with methoxy group and the response to DNA‐based sensor. Results may improve targeted extractions for specific compounds, leading to the achievement of extracts richer in antioxidant capacity, as well as in the tailoring of the biosensor response sensitivity to the composition of the extracts under analysis.
Goji extracts, obtained by MAE, were characterised through HPLC and their bioactive capacity was ascertained in terms of antimicrobial and antioxidant capacity, the later by spectrophotometric and electrochemical methods. |
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Bibliography: | FCT - No. UID/QUI/50006/2013; No. SFRH/BPD/78845/2011; No. SFRH/BPD/97049/2013 MCTES istex:810852D62861DE4E145AAD12EB79CA0B8FEBC7EB ArticleID:IJFS13105 FEDER funds through CCDR-N - No. NORTE-07-0124-FEDER-000069 ark:/67375/WNG-L3QMB9ND-J FSE Figure S1. HPLC chromatogram at 280 nm for L. barbarum extract number 2. Table S1. Analytical parameters from calibration curves used for HPLC-DAD quantification. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0950-5423 1365-2621 |
DOI: | 10.1111/ijfs.13105 |