NLRP3 played a role in Trichinella spiralis-triggered Th2 and regulatory T cells response

• Published in: Veterinary research (Paris) Vol. 51; no. 1; p. 107
• Main Authors: Jin, Xuemin, Bai, Xue, Yang, Yong, Ding, Jing, Shi, Haining, Fu, Baoquan, Boireau, Pascal, Liu, Mingyuan, Liu, Xiaolei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.08.2020; BioMed Central; BMC
• Subjects: Animals; Antibodies; Bone marrow; Bone morphogenetic proteins; Comparative analysis; Cytokines; dendritic cell; Dendritic cells; excretory-secretory products; Experiments; Female; Health aspects; Immune response; Infection; Infections; Larva - growth & development; Larva - physiology; Life Sciences; Lymphocytes; Medical research; Mice; Mice, Inbred C57BL; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; NLRP3; Parasites; Parasitic diseases; Proteins; Spleen; T cells; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - parasitology; Th2; Th2 Cells - immunology; Th2 Cells - parasitology; Transforming growth factors; Treg; Trichinella spiralis; Trichinella spiralis - growth & development; Trichinella spiralis - physiology; Trichinellosis - genetics; Trichinellosis - parasitology; United States > US; China; NLRP3; Trichinella spiralis; excretory-secretory products; dendritic cell; Treg; Th2
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-020-00829-2

Trichinella spiralis maintains chronic infections within its host. Muscle larvae excretory-secretory products (MLES) typically induce parasite-specific immune responses such as the Th2 response and regulatory T cells (Tregs) by modulating dendritic cell (DC) phenotype via the recognition of pattern recognition receptors (PRRs), such as Nod-like receptors (NLRs). We aimed to investigate the role of NLRP3 in T. spiralis-triggered immune response. We found that larvae burden was increased in NLRP3 mice compared to wild type (WT) mice. Administration of MLES induced higher levels of IL-4, IL-10, TGF-β and population of Tregs in WT mice than in NLRP3 mice. In vitro, we showed that increased expression of CD40 on the surface of MLES-treated DCs was inhibited after NLRP3 knockout. Increased production of IL-1β, IL-18, IL-10 and TGF-β, but not IL-12p70, was significantly diminished in the absence of NLRP3. Furthermore, our results demonstrated that MLES-treated DCs induced higher levels of IL-4, IL-10 and TGF-β and populations of Tregs in vitro. These inductions were abolished by NLRP3 deficiency in DCs, suggesting that NLRP3 in MLES-treated DCs plays a role in promoting the Th2 and Treg response. Taken together, we identified for the first time the involvement of NLRP3 in host defences against T. spiralis.