Axillary shoot proliferation and plant regeneration in Euryodendron excelsum H. T. Chang, a critically endangered species endemic to China

• Published in: Scientific reports Vol. 10; no. 1; p. 14402
• Main Authors: Chen, Shuangyan, Xiong, Yuping, Wu, Teng, Wu, Kunlin, da Silva, Jaime A. Teixeira, Xiong, Youhua, Zeng, Songjun, Ma, Guohua
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2020; Nature Publishing Group
• Subjects: 631/61; 631/80; Benzyl Compounds - pharmacology; Cell Proliferation - drug effects; China; Culture Media - chemistry; Endangered Species; Ericales - growth & development; Foreign trade zones; Humanities and Social Sciences; Macromolecules; Mathematical models; multidisciplinary; Naphthaleneacetic Acids - pharmacology; Plant Growth Regulators - pharmacology; Plant Roots - growth & development; Plant Shoots - growth & development; Purines - pharmacology; Regeneration - drug effects; Science; Science (multidisciplinary); Spatial distribution; Stochasticity; Synaptic transmission; Synaptic vesicles; Trees - growth & development; China
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-71360-9

Euryodendron excelsum H. T. Chang is a single-type, rare and endangered woody plant unique to China. In this study, young stems were used as explants and cultured on Woody Plant Medium (WPM) supplemented with 5.0 μM 6-benzyladenine (BA), were subcultured for more than 15 times over a total of more than 3 years and finally an efficient axillary shoot proliferation and plantlet regeneration system was established in which one shoot could proliferate an average of 5.1 axillary shoots every 2 months on the medium supplemented with 5.0 μM BA and 0.5 μM α-naphthaleneacetic acid (NAA). Shoots rooted at a moderate frequencies (50.1%) on agarized WPM supplemented with 0.5 μM NAA but 100% of shoots rooted in agar-free vermiculite-based WPM after culture for 2 months. Plantlets, when transplanted to peat soil: vermiculite (1:1), showed the highest 95.1% survival within 1 month.