Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans

• Published in: Scientific reports Vol. 10; no. 1; p. 55
• Main Authors: Lin, Shun Mei, Baek, Chan Yu, Jung, Jong-Hyun, Kim, Woo Sik, Song, Ha-Yeon, Lee, Ji Hee, Ji, Hyun Jung, Zhi, Yong, Kang, Bo Sun, Bahn, Yong-Sun, Seo, Ho Seong, Lim, Sangyong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.01.2020; Nature Publishing Group
• Subjects: 13/106; 13/2; 13/31; 13/95; 14/28; 631/326/252/953; 692/4017; 704/158/855; Animals; Antioxidants; Antioxidants - chemistry; Apoptosis; Apoptosis - drug effects; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms; Cell Line - cytology; Cell Line - drug effects; Cell Line - metabolism; Cell Wall - metabolism; Cell walls; Cosmetics; Deinococcus - metabolism; Deinococcus - radiation effects; Deinococcus radiodurans; Ethanol; Exopolysaccharides; Female; Gamma Rays; Humanities and Social Sciences; Humans; Keratinocytes; Mice; Mice, Inbred C57BL; Molecular weight; Monosaccharides; multidisciplinary; Mutation; Natural products; Polysaccharides, Bacterial - biosynthesis; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - pharmacology; Proteins; Radiation; Reactive Oxygen Species - chemistry; Reactive Oxygen Species - metabolism; Scanning electron microscopy; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-56141-3

Deinococcus radiodurans shows extreme resistance to a range of remarkable environmental stresses. Deinococcal exopolysaccharide (DeinoPol) is a component of the cell wall, but its role in stress resistance has not yet been well-described. In this study, we isolated and characterized DeinoPol from Deinococcus radiodurans R1 strain and investigated its application as an antioxidant agent. Bioinformatic analysis indicated that dra0033 , encoding an ExoP-like protein, was involved in DeinoPol biosynthesis, and dra0033 mutation significantly decreased survival rates in response to stresses. Purified DeinoPol consists of different monosaccharides and has a molecular weight of approximately 80 to 100 kDa. DeinoPol also demonstrates highly protective effects on human keratinocytes in response to stress-induced apoptosis by effectively scavenging ROS. Taken together, these findings indicate that DeinoPol is the first reported deinococcal exopolysaccharide that might be used in cosmetics and pharmaceuticals as a safe and attractive radical scavenger.