Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells

• Published in: Scientific reports Vol. 10; no. 1; p. 13690
• Main Authors: Huang, Denggao, Gao, Yuanhui, Wang, Shunlan, Zhang, Wei, Cao, Hui, Zheng, Linlin, Chen, Yang, Zhang, Shufang, Chen, Jie
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.08.2020; Nature Publishing Group
• Subjects: 639/166/985; 639/166/987; Adipose Tissue - chemistry; Adipose Tissue - cytology; Adipose Tissue - radiation effects; Apoptosis; c-Myc protein; Cell cycle; Cell Differentiation - radiation effects; Cell growth; Cell proliferation; Cell Proliferation - radiation effects; Cell surface; Cell Survival - radiation effects; Cells, Cultured; Cytokines; Enzyme-linked immunosorbent assay; Experiments; Flow Cytometry; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation - radiation effects; Gene Regulatory Networks - radiation effects; Genes; Humanities and Social Sciences; Humans; Mesenchymal stem cells; Mesenchymal Stem Cells - chemistry; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - radiation effects; Mesenchyme; Metabolites; Metabolomics; Metabolomics - methods; multidisciplinary; Myc protein; Paracrine signalling; Polymerase chain reaction; Science; Science (multidisciplinary); Stromal cells; Surface markers; Transcription; Ultrasonic imaging; Ultrasonic Waves; Ultrasound
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-69430-z

To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the proliferation of human adipose-derived mesenchymal stromal cells (hASCs) and uncovered its stimulation mechanism. LIPUS at 30 mW/cm 2 was applied for 5 min/day to promote the proliferation of hASCs. Flow cytometry was used to study the cell surface markers, cell cycle, and apoptosis of hASCs. The proliferation of hASCs was detected by cell counting kit-8, cell cycle assay, and RT-PCR. The expression of hASCs cytokines was determined by ELISA. The differences between transcriptional genes and metabolites were analyzed by transcript analysis and metabolomic profiling experiments. The number of cells increased after LIPUS stimulation, but there was no significant difference in cell surface markers. The results of flow cytometry, RT-PCR, and ELISA after LIPUS was administered showed that the G 1 and S phases of the cell cycle were prolonged. The expression of cell proliferation related genes ( CyclinD1 and c-myc ) and the paracrine function related gene ( SDF-1α ) were up-regulated. The expression of cytokines was increased, while the apoptosis rate was decreased. The results of transcriptome experiments showed that there were significant differences in 27 genes;15 genes were up-regulated, while 12 genes were down-regulated. The results of metabolomics experiments showed significant differences in 30 metabolites; 7 metabolites were up-regulated, and 23 metabolites were down-regulated. LIPUS at 30 mW/cm 2 intensity can promote the proliferation of hASCs cells in an undifferentiating state, and the stem-cell property of hASCs was maintained. CyclinD1 gene, c-myc gene, and various genes of transcription and products of metabolism play an essential role in cell proliferation. This study provides an important experimental and theoretical basis for the clinical application of LIPUS in promoting the proliferation of hASCs cells.