Silkworm Gut Fiber of Bombyx mori as an Implantable and Biocompatible Light-Diffusing Fiber

• Published in: International journal of molecular sciences Vol. 17; no. 7; p. 1142
• Main Authors: Cenis, Jose Luis, Aznar-Cervantes, Salvador D, Lozano-Pérez, Antonio Abel, Rojo, Marta, Muñoz, Juan, Meseguer-Olmo, Luis, Arenas, Aurelio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.07.2016; MDPI
• Subjects: Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Bombyx - metabolism; Bombyx mori; Cell Proliferation - radiation effects; Cells, Cultured; Diffusion; Fibers; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - radiation effects; Gastrointestinal Tract - metabolism; Light; light-diffusing optical fiber; Mice; Prostheses and Implants; Silk - chemistry; Silk - metabolism; silk fibroin; silkworm gut fiber; Tensile Strength - radiation effects; Transplants & implants; Worms; light-diffusing optical fiber; biocompatibility; silkworm gut fiber; silk fibroin
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms17071142

This work describes a new approach to the delivery of light in deeper tissues, through a silk filament that is implantable, biocompatible, and biodegradable. In the present work, silkworm gut fibers (SGFs) of Bombyx mori L., are made by stretching the silk glands. Morphological, structural, and optical properties of the fibers have been characterized and the stimulatory effect of red laser light diffused from the fiber was assayed in fibroblast cultures. SGFs are formed by silk fibroin (SF) mainly in a β-sheet conformation, a stable and non-soluble state in water or biological fluids. The fibers showed a high degree of transparency to visible and infrared radiation. Using a red laser (λ = 650 nm) as source, the light was efficiently diffused along the fiber wall, promoting a significant increment in the cell metabolism 5 h after the irradiation. SGFs have shown their excellent properties as light-diffusing optical fibers with a stimulatory effect on cells.