Innovative techniques, sensors, and approaches for imaging biofilms at different scales

Highlights • This review discusses advanced confocal laser-scanning microscopy of biofilms (probes, hardware, and data analysis). • How biofilm assessment can be carried out at the nanoscale by SIM, STED, and blink microscopy. • How biofilm imaging can be performed at the mesoscale using optical coh...

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Bibliographic Details
Published inTrends in microbiology (Regular ed.) Vol. 23; no. 4; pp. 233 - 242
Main Authors Neu, Thomas R, Lawrence, John R
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2015
Subjects
biofilm
Biofilms
Internal Medicine
laser microscopy
mesoscale
microscale
Microscopy - methods
Microscopy, Confocal - methods
Molecular Imaging - methods
nanoscale
sensors
Tomography, Optical Coherence - methods
microscale
laser microscopy
nanoscale
sensors
mesoscale
biofilm
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Summary:Highlights • This review discusses advanced confocal laser-scanning microscopy of biofilms (probes, hardware, and data analysis). • How biofilm assessment can be carried out at the nanoscale by SIM, STED, and blink microscopy. • How biofilm imaging can be performed at the mesoscale using optical coherence tomography. • Visualization, quantification, and deconvolution by digital image analysis is also explored.
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ObjectType-Review-1
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ISSN:0966-842X
1878-4380
DOI:10.1016/j.tim.2014.12.010