Ribosomal protein S3 regulates GLI2-mediated osteosarcoma invasion

• Published in: Cancer letters Vol. 356; no. 2; pp. 855 - 861
• Main Authors: Nagao-Kitamoto, Hiroko, Setoguchi, Takao, Kitamoto, Sho, Nakamura, Shunsuke, Tsuru, Arisa, Nagata, Masahito, Nagano, Satoshi, Ishidou, Yasuhiro, Yokouchi, Masahiro, Kitajima, Shinichi, Yoshioka, Takako, Maeda, Shingo, Yonezawa, Suguru, Komiya, Setsuro
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 28.01.2015; Elsevier Limited
• Subjects: Adolescent; Adult; Antigens; Apoptosis; Blotting, Western; Bone and Bones - metabolism; Bone and Bones - pathology; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Bone Neoplasms - pathology; Cell Adhesion; Cell Movement; Cell Proliferation; Child; DNA repair; Experiments; Female; Gene Expression Regulation, Neoplastic; GLI2; Hedgehog; Hematology, Oncology and Palliative Medicine; Humans; Immunoenzyme Techniques; Kruppel-Like Transcription Factors - genetics; Kruppel-Like Transcription Factors - metabolism; Laboratories; Male; Medical prognosis; Metastasis; Middle Aged; Neoplasm Invasiveness; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - pathology; Patients; Promoter Regions, Genetic - genetics; Proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal protein S3 (RPS3); Ribosomal Proteins - antagonists & inhibitors; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Studies; Tumor Cells, Cultured; Young Adult; Zinc Finger Protein Gli2; Japan; RNA interference; GLI; arsenic trioxide; recombinant sonic hedgehog; β-actin; ACTB; normal human osteoblast cells; GLI2; DMEM; Tris-buffered saline Tween-20; Smoothened; suppressor of fused; rSHH; Osteosarcoma; SUFU; FBS; glioma-associated oncogene homolog; RPS3; sodium dodecyl sulfate; NHOst; RNAi; SDS; PTCH1; TBST; Dulbecco's modified Eagle's medium; fetal bovine serum; Hedgehog; SMO; ribosomal protein S3; Patched1; ATO; Ribosomal protein S3 (RPS3)
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2014.10.042

Highlights • RPS3 was identified as a novel downstream gene of GLI2. • Knockdown of GLI2 decreased RPS3 expression in osteosarcoma cells. • RPS3 knockdown decreased the migration and invasion of osteosarcoma cells. • RPS3 expression was increased in primary osteosarcoma lesions with lung metastases. • GLI2–RPS3 pathway may be an attractive therapeutic target for treating osteosarcoma metastasis.