Protective effect of vaccination with recombinant proteins from Streptococcus equi subspecies equi in a strangles model in the mouse

• Published in: Vaccine Vol. 24; no. 19; pp. 4144 - 4151
• Main Authors: Flock, M., Karlström, Å., Lannergård, J., Guss, B., Flock, J.-I.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 08.05.2006; Elsevier
• Subjects: Animals; Antigens, Bacterial - genetics; Applied microbiology; Bacteriology; Base Sequence; Biological and medical sciences; Collagen - immunology; Disease Models, Animal; DNA, Bacterial - genetics; Extra cellular proteins; Fundamental and applied biological sciences. Psychology; Horse Diseases - immunology; Horse Diseases - prevention & control; Horses; Medicin och hälsovetenskap; Mice; Microbiology; Miscellaneous; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Strangles; Streptococcal Infections - immunology; Streptococcal Infections - prevention & control; Streptococcal Infections - veterinary; Streptococcal Vaccines - genetics; Streptococcal Vaccines - pharmacology; Streptococcus equi; Streptococcus equi - genetics; Streptococcus equi - immunology; Streptococcus equi - pathogenicity; Vaccination; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Subunit - genetics; Vaccines, Subunit - pharmacology; Vaccines, Synthetic - genetics; Vaccines, Synthetic - pharmacology; Strangles; Streptococcus equi; Extra cellular proteins; Vaccination; Animal model; Infection; Streptococcaceae; Streptococcal infection; Streptococcus A; Bacteriosis; Bacteria; Micrococcales; Recombinant protein; Veterinary
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2006.02.016

A mouse model resembling Streptococcus equi subspecies equi infection in the horse, strangles, was used to assess the protective effect of vaccination with selected recombinant proteins from S. equi subsp. equi. After challenge the infection was monitored by weight loss and by nasal colonisation with S. equi subsp. equi. Vaccination with a collagen-binding protein (CNE) and a collagen-like protein (SclC) resulted in protective antibodies, whereas a novel fibronectin-binding protein (FNEB) did not. Co-administration of CNE with EAG, a poorly immunogenic α 2-macroglobulin-, albumin- and immunoglobulin G-binding protein, resulted in a significant synergistic effect and enhanced the protective immune response against EAG.