Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Study

• Published in: Scientific reports Vol. 8; no. 1; pp. 17620 - 15
• Main Authors: Onak, Günnur, Şen, Mustafa, Horzum, Nesrin, Ercan, Utku Kürşat, Yaralı, Ziyşan Buse, Garipcan, Bora, Karaman, Ozan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.12.2018; Nature Publishing Group
• Subjects: 13/100; 13/107; 14; 14/1; 14/28; 14/34; 140/146; 38; 639/166/985; 639/301/54/2295; 639/301/54/989; 639/301/54/990; 639/925/352/1061; Acids; Alkaline phosphatase; Aspartic acid; Biomimetics; Bone marrow; Bone matrix; Calcium; Collagen (type I); Comparative studies; Contact angle; Extracellular matrix; Fourier transforms; Glutamic acid; Glycolic acid; Humanities and Social Sciences; Infrared spectroscopy; Mesenchymal stem cells; Mesenchyme; Mineralization; multidisciplinary; Osteocalcin; Osteopontin; Peptides; Photoelectron spectroscopy; Polylactide-co-glycolide; Scanning electron microscopy; Science; Science (multidisciplinary); Spectrum analysis; Stem cells; Surface properties; Cold Atmospheric Plasma (CAP); Peptide Conjugates; Water Contact Angle; Osteogenic Differentiation; Nanofibers (NF)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-36109-5

Optimization of nanofiber (NF) surface properties is critical to achieve an adequate cellular response. Here, the impact of conjugation of biomimetic aspartic acid (ASP) and glutamic acid (GLU) templated peptides with poly(lactic-co-glycolic acid) (PLGA) electrospun NF on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) was evaluated. Cold atmospheric plasma (CAP) was used to functionalize the NF surface and thus to mediate the conjugation. The influence of the CAP treatment following with peptide conjugation to the NF surface was assessed using water contact angle measurements, Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS). The effect of CAP treatment on morphology of NF was also checked using Scanning Electron Microscopy (SEM). Both the hydrophilicity of NF and the number of the carboxyl (-COOH) groups on the surface increased with respect to CAP treatment. Results demonstrated that CAP treatment significantly enhanced peptide conjugation on the surface of NF. Osteogenic differentiation results indicated that conjugating of biomimetic ASP templated peptides sharply increased alkaline phosphatase (ALP) activity, calcium content, and expression of key osteogenic markers of collagen type I (Col-I), osteocalcin (OC), and osteopontin (OP) compared to GLU conjugated (GLU-pNF) and CAP treated NF (pNF). It was further depicted that ASP sequences are the major fragments that influence the mineralization and osteogenic differentiation in non-collagenous proteins of bone extracellular matrix.