Development of Highly Sensitive Analytical Methods for Biologically Relevant Materials and Their Pharmaceutical Applications

• Published in: Chemical & pharmaceutical bulletin Vol. 65; no. 12; pp. 1099 - 1112
• Main Author: Arakawa, Hidetoshi
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 2017; Japan Science and Technology Agency
• Subjects: Analytical chemistry; Analytical methods; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - pharmacology; Biological properties; Biological samples; Bioluminescence; Biomarkers, Tumor - analysis; Capillary electrophoresis; Chemiluminescence; Chemistry, Pharmaceutical - methods; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA - analysis; DNA probe; Electrophoresis; enzyme immunoassay; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Hormones; Humans; Hybridization; Immunoassay - methods; Immunoassays; Luminescent Measurements; Mathematical analysis; Medical treatment; Organic chemistry; Pharmaceutical Preparations - analysis; Pharmaceuticals; reactive oxygen; Reactive oxygen species; Reactive Oxygen Species - analysis; Tumor markers; enzyme immunoassay; reactive oxygen; bioluminescence; chemiluminescence; DNA probe; capillary electrophoresis
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.c17-00787

One important aspect of analytical chemistry research in the pharmaceutical sciences is the development of diagnostic and therapeutic analyses for disease, and the development of analytical methods for elucidating the causes of disease. I have been focusing on developing a highly sensitive method for measuring trace amounts of specific components in biological samples. This research can be roughly divided into three approaches: the use of immunoassays and DNA hybridization as methods utilizing specific affinities, the use of capillary electrophoresis as a highly sensitive and rapid separation method, and the use of chemiluminescence and bioluminescence reactions. The components being measured are compounds such as hormones, tumor markers, drugs, reactive oxygen species and genes in biological samples for the purpose of developing therapies for the prevention and treatment of diseases.