Antagonism between two root-associated beneficial Pseudomonas strains does not affect plant growth promotion and induced resistance against a leaf-chewing herbivore

• Published in: FEMS microbiology ecology Vol. 93; no. 4; p. 1
• Main Authors: Pangesti, Nurmi, Vandenbrande, Simon, Pineda, Ana, Dicke, Marcel, Raaijmakers, Jos M., Van Loon, Joop J.A.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2017
• Subjects: Agricultural pests; Antagonism; Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins; Arabidopsis thaliana; Botanical research; Chewing; Diseases and pests; Ecology; Gene Expression Regulation, Plant; Herbivores; Herbivory; Host plants; Host-bacteria relationships; Inoculation; Insect resistance; Insect-plant relationships; Leaves; Lepidoptera; Mamestra brassicae, roots; Mastication; microbe–plant–insect interactions; microbial antagonism; Microbiology; Physiological aspects; Plant biomass; Plant Development; Plant growth; Plant Leaves - metabolism; Plant Roots - metabolism; Plant Roots - physiology; Plants; Population density; Promotion; Pseudomonas; Pseudomonas - metabolism; Pseudomonas - physiology; Pseudomonas fluorescens; Pseudomonas fluorescens - growth & development; Rhizosphere; Roots; Symbiosis; Tips; Netherlands; microbial antagonism; microbe–plant–insect interactions; roots; Arabidopsis thaliana; Mamestra brassicae, roots
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fix038

Abstract Plant growth-promoting microbes residing on the roots may cooperate or compete, thereby affecting their collective benefit to the host plant. Pseudomonas simiae WCS417r (formerly known as P. fluorescens WCS417r) and Pseudomonas fluorescens SS101 are well known for their ability to induce systemic resistance in Arabidopsis. Here, we evaluate how these species interact on the roots of Arabidopsis thaliana Col-0 and how their co-inoculation affects plant defense to the leaf-chewing herbivore Mamestra brassicae and plant growth promotion. WCS417r and SS101, applied individually to root tips or at two different positions along the roots, established similar population densities on Arabidopsis roots. When co-inoculated at the same position on the roots, however, WCS417r established significantly higher population densities than SS101. Both upon single inoculation and co-inoculation, the two pseudomonads induced the same level of induced systemic resistance against the caterpillar M. brassicae and the same increase in plant biomass. These results suggest that combined inoculation of both Pseudomonas strains does not significantly modify the plant's defensive capacity compared to individual inoculation, resulting in a similar effect on performance of the generalist herbivore M. brassicae. Antagonism between two root-associated beneficial Pseudomonas strains does not affect the strength of plant growth promotion and induced systemic resistance against a leaf-chewing herbivore.