Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription...

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Published inOpen forum infectious diseases Vol. 9; no. 11; p. ofac619
Main Authors Su, Maxwell, Ping, Sara, Nguyen, Phuong-Vi, Rojas, Alejandra, Hussaini, Laila, Carmola, Ludy Registre, Taz, Azmain, Sullivan, Julie, Martin, Greg S, Piantadosi, Anne, Martinez, Magaly, Lam, Wilbur A, Anderson, Evan J, Waggoner, Jesse J
Format Journal Article
LanguageEnglish
Published US Oxford University Press 01.11.2022
Subjects
Brief Report
severe acute respiratory syndrome coronavirus 2
variants of concern
subgenomic RNA
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Summary:Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant.
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Potential conflicts of interest. All authors: No reported conflicts of interest.
ISSN:2328-8957
2328-8957
DOI:10.1093/ofid/ofac619