Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleoti...

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Bibliographic Details
Published inNucleic acids research Vol. 37; no. 20; pp. 6984 - 6990
Main Author Gibson, Daniel G.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.11.2009
Subjects
DNA - biosynthesis
DNA - chemistry
Oligonucleotides - chemistry
Saccharomyces cerevisiae - genetics
Synthetic Biology and Chemistry
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Summary:Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleotides in length. This straightforward scheme for assembling chemically-synthesized oligonucleotides could be a useful tool for building synthetic DNA molecules.
Bibliography:ark:/67375/HXZ-64F19G0K-S
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ArticleID:gkp687
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkp687