Effects of rapeseed oil on activity of methylmalonyl-CoA carboxyltransferase in culture of Streptomyces fradiae

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 5; pp. 902 - 906
• Main Authors: Choi, D.B. (Gifu Univ. (Japan)), Park, Y, Okabe, M
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.1998; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACEITE DE COLZA; ACIDE PROPIONIQUE; ACIDO PROPIONICO; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Antibiotics (antibacterial agents, antifungal agents); Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Applied microbiology; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Biotechnology; CARBON; CARBONE; CARBONO; ENZYMIC ACTIVITY; Fundamental and applied biological sciences. Psychology; HUILE DE COLZA; methylmalonyl-CoA carboxyltransferase; Microbiology; Mission oriented research; Physiology and metabolism; PROPIONIC ACID; propoinic acid; RAPESEED OIL; STREPTOMYCES; Streptomyces fradiae; TILOSINA; TRANSFERASAS; TRANSFERASE; TRANSFERASES; TYLOSIN; TYLOSINE; Culture medium; Streptomycetaceae; Streptomyces fradiae; Enzyme; Transferases; Propionic acid; Biosynthesis; Carbon; Macrolide; Methylmalonyl-CoA carboxyltransferase; Actinomycetales; Rapeseed oil; Antibiotic; Enzymatic activity; Nutrient source; Microorganism culture; Bacteria; Actinomycetes; Tylosin; Antibacterial agent
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.62.902

To investigate why more tylosin was produced when Streptomyces fradiae T1558 was cultured in a rapeseed oil medium than in a glucose or starch medium, we measured the activity of methylmalonyl-CoA carboxyltransferase (EC 2.1.3.1) and intracellular propionic acid. The activity of the enzyme, which catalyzes the formation of the precursor of tylosin, protylonolide, was 0.19 U/mg protein in 5 days of culture in rapeseed oil medium, which was 2.5- and 1.3-fold that with the glucose or starch medium, respectively. The intracellular propionic acid concentration was 1.2 g/g of days weight, which was 4.3- and 2.1-fold that with the glucose or starch medium, respectively. The addition of propionic acid increased tylosin production in batch culture: when 0.2 g/l (final concentration) propionic acid was added to the glucose medium, 3.8 g/l tylosin was produced in 10 days of culture, 4.7-fold the amount without propionic acid. These findings suggest that in glucose medium, intracellular propionic acid is a limiting factor because of the low activity of methylmalonyl-CoA carboxyltransferase of the tylosin biosynthesis pathway