Mutational analysis of the Drosophila DNA repair and recombination gene mei-9

• Published in: Genetics (Austin) Vol. 167; no. 1; pp. 263 - 273
• Main Authors: Yildiz, O, Kearney, H, Kramer, B.C, Sekelsky, J.J
• Format: Journal Article
• Language: English
• Published: United States Genetics Soc America 01.05.2004; Genetics Society of America
• Subjects: Alleles; Amino Acid Motifs; Amino Acid Sequence; Animals; Blotting, Western; Codon, Nonsense; Deoxyribonucleic acid; DNA; DNA Damage; DNA Mutational Analysis; DNA Repair; Drosophila; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; Drosophila Proteins - physiology; Frameshift Mutation; Gene Deletion; Genetics; Insects; Meiosis; Models, Genetic; Molecular Sequence Data; Mutation; Mutation, Missense; Nuclear Proteins - genetics; Nuclear Proteins - physiology; Proteins; Recombination, Genetic; Sequence Homology, Amino Acid; Two-Hybrid System Techniques; Ultraviolet Rays
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1534/genetics.167.1.263

Drosophila mei-9 is essential for several DNA repair and recombination pathways, including nucleotide excision repair (NER), interstrand crosslink repair, and meiotic recombination. To better understand the role of MEI-9 in these processes, we characterized 10 unique mutant alleles of mei-9. These include a P-element insertion that disrupts repair functions but not the meiotic function; three nonsense mutations, one of which has nearly wild-type levels of protein; three missense mutations, one of which disrupts the meiotic function but not repair functions; two small in-frame deletions; and one frameshift.