Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil

• Published in: Scientific reports Vol. 9; no. 1; p. 4134
• Main Authors: Chaudhary, Dhiraj Kumar, Kim, Dong-Uk, Kim, Dockyu, Kim, Jaisoo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.03.2019; Nature Publishing Group
• Subjects: 101/58; 45/22; 45/23; 45/29; 45/77; 631/326/1320; 704/172/4081; Arctic Regions; Biotransformation; Deoxyribonucleic acid; Diesel; DNA; Flavobacterium; Flavobacterium - classification; Flavobacterium - genetics; Flavobacterium - metabolism; Gasoline - microbiology; Genome, Bacterial; Humanities and Social Sciences; Hybridization; multidisciplinary; New species; Nucleotide sequence; Oil; Phylogenetics; Phylogeny; rRNA 16S; Science; Science (multidisciplinary); Sequence Homology, Nucleic Acid; Soil Microbiology; Soil Pollutants - metabolism; Soil pollution; Arctic Regions; Arctic region
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-40667-7

This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42 T and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium , with the nearest relative being Flavobacterium psychrolimnae LMG 22018 T (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42 T and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42 T and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42 T and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42 T and nearest relative F . psychrolimnae LMG 22018 T were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42 T belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42 T from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42 T and Kopri-43 represents a single novel species of the genus Flavobacterium , for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42 T (=KEMB 9005-710 T  = KACC 19625 T  = NBRC 113374 T ).