Magnetic forces enable rapid endothelialization of synthetic vascular grafts

• Published in: Circulation (New York, N.Y.) Vol. 114; no. 1; pp. I314 - I318
• Main Authors: PISLARU, Sorin V, HARBUZARIU, Adriana, AGARWAL, Gautam, WITT, Tyra, GULATI, Rajiv, SANDHU, Nicole P, MUESKE, Cheryl, KALRA, Manju, SIMARI, Robert D, SANDHU, Gurpreet S
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 04.07.2006
• Subjects: Animals; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Blood Vessel Prosthesis; Blood Vessel Prosthesis Implantation; Cardiology. Vascular system; Cell Adhesion; Cell Survival; Cells, Cultured - cytology; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Endocytosis; Endothelial Cells - cytology; Endothelium, Vascular - cytology; Equipment Design; Ferric Compounds; Graft Occlusion, Vascular - prevention & control; Implants, Experimental; Magnetics; Medical sciences; Microscopy, Confocal; Microscopy, Electron; Microspheres; Polyethylene Terephthalates; Pulsatile Flow; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the heart; Sus scrofa; Endothelialization; coronary disease; Surgery; grafting; Cardiovascular disease; Coronary heart disease; magnet; Endothelium
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circulationaha.105.001446

Synthetic vascular grafts cannot be used in small vessels because of graft failure caused by thrombosis and neointima formation. Rapid endothelialization may overcome this limitation. We hypothesized that a magnetic graft would be able to capture and retain endothelial cells labeled with paramagnetic particles. Porcine blood derived endothelial cells were allowed to endocytose superparamagnetic iron oxide microspheres. Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent cell survival of 75% to 95%. A flexible magnetic sheet was annealed to the external surface of a knitted Dacron graft. Labeled cells (10(6)/mL) were placed within the graft for 5 minutes. Confocal and electron microscopy confirmed uniform cell capture at the magnetized surface. The effect of shear forces on the adherent cells was evaluated in a flow chamber. The cells remained attached at rates up to 300 mL/min, with cell loss commencing at 400 mL/min. Prototype magnetic grafts were implanted in porcine carotid arteries. Labeled cells were placed within the graft for 10 minutes at the time of implantation. The grafts were evaluated after one day and uniform cell coverage was noted on the magnetized surface. In comparison, relatively few labeled cells were seen attached to a nonmagnetized surface. Magnetic forces can be used to rapidly cover a vascular graft with paramagnetically labeled cells. This biophysical interaction is sufficient to retain cells in the presence of blood flow. Applications of this technique may include rapid endothelialization of synthetic vascular grafts and dialysis fistulas.