Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

• Published in: Scientific reports Vol. 10; no. 1; p. 11845
• Main Authors: Rubio-del-Campo, Antonio, Alcántara, Cristina, Collado, María Carmen, Rodríguez-Díaz, Jesús, Yebra, María J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.07.2020; Nature Publishing Group
• Subjects: 631/326; 631/337; Acetates - metabolism; Acetic acid; Acetylglucosamine - analogs & derivatives; Acetylglucosamine - isolation & purification; Baby foods; Bifidobacterium bifidum - classification; Bifidobacterium bifidum - genetics; Bifidobacterium bifidum - isolation & purification; Bifidobacterium bifidum - metabolism; Bifidobacterium breve - classification; Bifidobacterium breve - genetics; Bifidobacterium breve - isolation & purification; Bifidobacterium breve - metabolism; Breast milk; Carbohydrates; Clostridiales - classification; Clostridiales - genetics; Clostridiales - isolation & purification; Clostridiales - metabolism; Disaccharidases - isolation & purification; Disaccharides; Disaccharides - isolation & purification; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Enterobacteriaceae - classification; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; Enterobacteriaceae - metabolism; Fecal microflora; Feces; Feces - microbiology; Fermentation; Formates - metabolism; Gastrointestinal Microbiome - physiology; Humanities and Social Sciences; Humans; Infant; Infants; Intestinal microflora; Lactic acid; Lactic Acid - metabolism; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus - metabolism; Microbiota; Milk; Milk, Human - chemistry; Mucosa; multidisciplinary; Oligosaccharides; Prebiotics - analysis; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-68718-4

Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto- N -biose (LNB), galacto- N -biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum . The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.