Effectiveness of disinfectant solutions associated or not with brushing on the biofilm control of a 3D printed-denture base resin

• Published in: Journal of applied oral science Vol. 31; p. e20230104
• Main Authors: Nunes, Thaís Soares Bezerra Santos, Silva, Marcela Dantas Dias da, Coelho, Sabrina Romão Gonçalves, Viotto, Hamile Emanuella do Carmo, Pero, Ana Carolina
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade De Odontologia De Bauru - USP 01.01.2023; University of São Paulo
• Subjects: Biofilms; Candida albicans; DENTISTRY, ORAL SURGERY & MEDICINE; Denture Bases; Denture cleansers; Disinfectants; Humans; Materials Testing; Original; Printing, Three-Dimensional; Surface Properties; Denture bases; Biofilms; Denture cleansers; Printing, Three-Dimensional
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/1678-7757-2023-0104

The formation of biofilm on denture bases is a recurrent clinical problem that favors the development of denture stomatitis. The effectiveness of a hygiene protocol in a 3D-printed denture base resin is still uncertain. To evaluate of the effectiveness of immersion, associated or not with brushing in a soap solution, on the biofilm control of a 3D-printed denture base resin. Specimens of denture base resins [Cosmos Denture (COS) and Classico (CLA/control)] were contaminated in vitro with Candida albicans and immersed in sodium hypochlorite 0.25% (SH, alkaline peroxide) AP, chlorhexidine digluconate 2% (CD or PBS-Control), associated or not with brushing with 0.78% Lifebuoy soap. Roughness was evaluated before and after brushing and immersion. The effectiveness of the protocols was assessed by CFU/mL, cellular metabolism (XTT), scanning electron microscopy (SEM), and confocal scanning laser microscopy. Data were analyzed by T student, ANOVA/Welch, and Tukey/Gomes-Howell pos-hoc tests (α = 0.05). CLA showed greater roughness than COS. CFU/mL and XTT were higher in COS resin with a higher hyphae formation. Immersion in SH and CD eliminated CFU/mL and reduced XTT for both resins, associated or not with brushing. AP reduced CFU/mL only when associated with brushing. The biofilm on the 3D-printed resin was thicker and presumably more pathogenic, regardless of its smoother surface. Immersions in SH 0.25% and CD 2% are effective hygiene protocols for both resins, associated or not with brushing. AP should be recommended when associated with brushing with a Lifebuoy 0.78% solution.