Retinoblastoma protein-interacting zinc finger 1 (RIZ1) participates in RANKL-induced osteoclast formation via regulation of NFATc1 expression

• Published in: Immunology letters Vol. 131; no. 2; pp. 166 - 169
• Main Authors: Noman, Abu Shadat Mohammod, Koide, Naoki, Iftakhar-E-Khuda, Imtiaz, Dagvadorj, Jargalsaikhan, Tumurkhuu, Gantsetseg, Naiki, Yoshikazu, Komatsu, Takayuki, Yoshida, Tomoaki, Yokochi, Takashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 08.07.2010
• Subjects: Acid phosphatase (tartrate-resistant); Acid Phosphatase - biosynthesis; Allergy and Immunology; Animals; Antigens, Differentiation - biosynthesis; Bone Resorption; Cell Line; Gene Expression Regulation; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - immunology; Histone-Lysine N-Methyltransferase - metabolism; Isoenzymes - biosynthesis; Macrophages - immunology; Macrophages - metabolism; Macrophages - pathology; Mice; Neoplasms - metabolism; Neoplasms - pathology; Neoplasms - physiopathology; NFATC Transcription Factors - biosynthesis; NFATC Transcription Factors - genetics; NFATc1; Osteoclastogenesis; Osteoclasts - immunology; Osteoclasts - metabolism; Osteoclasts - pathology; RANK Ligand - metabolism; RANKL; RAW 264.7; RIZ1; RNA, Small Interfering - genetics; Signal Transduction - genetics; Signal Transduction - immunology; Tartrate-Resistant Acid Phosphatase; Transcription Factors - genetics; Transcription Factors - immunology; Transcription Factors - metabolism; Osteoclastogenesis; RIZ1; RANKL; NFATc1; RAW 264.7
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2010.04.006

Abstract The role of retinoblastoma protein-interacting zinc finger 1 (RIZ1) in receptor activator of NF-κB ligand (RANKL)-induced osteoclast formation was examined in mouse RAW 264.7 macrophage-like cells. The expression of RIZ1 was significantly augmented by RANKL-treated cells. Silencing of RIZ1 with the siRNA significantly reduced the appearance of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells as osteoclasts in RANKL-treated cells. The expression of nuclear factor of activated T cell 1 (NFATc1) as the terminal transcription factor of osteoclast formation was prevented by RIZ1 siRNA. It was suggested that that RIZ1 might participate in RANKL-induced osteoclast formation through the regulation of NFATc1 expression.