Phage delivered CRISPR-Cas system to combat multidrug-resistant pathogens in gut microbiome

• Published in: Biomedicine & pharmacotherapy Vol. 151; p. 113122
• Main Authors: Nath, Arijit, Bhattacharjee, Rahul, Nandi, Aditya, Sinha, Adrija, Kar, Sulagna, Manoharan, Nikita, Mitra, Shirsajit, Mojumdar, Abhik, Panda, Pritam Kumar, Patro, Swadheena, Dutt, Ateet, Ahuja, Rajeev, Verma, Suresh K., Suar, Mrutyunjay
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.07.2022; Elsevier
• Subjects: Bacteriophages; CRISPR-Cas system; Gut microbiome; MDR pathogen; FliD; FliC; GFPT; RNA; CarO; CRISPR-Cas system; ermB; LPS; CRISPR-Cas; ISC; MDR; TLR; Bacteriophages; AMR; Tn5398; MDR pathogen; GIT; CrRNA; OmpC; DNA; ROS; Cwp66; PAM; Gut microbiome; FimH
• ISSN: 0753-3322; 1950-6007; 1950-6007
• DOI: 10.1016/j.biopha.2022.113122

The Host-microbiome interactions that exist inside the gut microbiota operate in a synergistic and abnormal manner. Additionally, the normal homeostasis and functioning of gut microbiota are frequently disrupted by the intervention of Multi-Drug Resistant (MDR) pathogens. CRISPR-Cas (CRISPR-associated protein with clustered regularly interspersed short palindromic repeats) recognized as a prokaryotic immune system has emerged as an effective genome-editing tool to edit and delete specific microbial genes for the expulsion of bacteria through bactericidal action. In this review, we demonstrate many functioning CRISPR-Cas systems against the anti-microbial resistance of multiple pathogens, which infiltrate the gastrointestinal tract. Moreover, we discuss the advancement in the development of a phage-delivered CRISPR-Cas system for killing a gut MDR pathogen. We also discuss a combinatorial approach to use bacteriophage as a delivery system for the CRISPR-Cas gene for targeting a pathogenic community in the gut microbiome to resensitize the drug sensitivity. Finally, we discuss engineered phage as a plausible potential option for the CRISPR-Cas system for pathogenic killing and improvement of the efficacy of the system. [Display omitted]