Characterization of antennal chemosensilla and associated odorant binding as well as chemosensory proteins in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)

• Published in: Scientific reports Vol. 8; no. 1; pp. 7649 - 11
• Main Authors: Wang, Shan-Ning, Shan, Shuang, Liu, Jing-Tao, Li, Rui-Jun, Lu, Zi-Yun, Dhiloo, Khalid Hussain, Khashaveh, Adel, Zhang, Yong-Jun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.05.2018; Nature Publishing Group
• Subjects: 14; 14/63; 38; 38/32; 631/337/572; 631/601/1466; Antennae; Antennal sensilla; Chemoreception; Humanities and Social Sciences; Hymenoptera; Microplitis mediator; Morphology; multidisciplinary; Odorant-binding protein; Olfactory perception; Parasitoids; Physical characteristics; Pores; Proteins; Science; Science (multidisciplinary); Sensilla
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-25996-3

Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) expressed in antennal chemosensilla are believed to be important in insect chemoreception. In the current study, we fully described the morphological characteristics of the antennal sensilla in parasitoid wasp Microplitis mediator and analyzed the expression patterns of OBPs and CSPs within the antennae. In M. mediator , eight types of sensilla were observed on the antennae. Sensilla basiconica type 2 and s. placodea with wall pores may be involved in olfactory perception, whereas s. basiconica type 1 and type 3 with tip pores may play gustatory functions. Among the 18 OBPs and 3 CSPs in M. mediator , 10 OBPs and 2 CSPs were exclusively or primarily expressed in the antennae. In situ hybridization experiments indicated that the 12 antennae-enriched OBPs and CSPs were mapped to five morphological classes of antennal sensilla, including s. basiconica (type 1–3), s. placodea and s. coeloconica. Within the antennae, most of OBP and CSP genes were expressed only in one type of sensilla indicating their differentiated roles in detection of special type of chemical molecules. Our data will lay a foundation to further study the physiological roles of OBPs and CSPs in antennae of parasitoid wasps.