A Novel Rapid Fungal Promoter Analysis System Using the Phosphopantetheinyl Transferase Gene, npgA , in Aspergillus nidulans

• Published in: Mycobiology Vol. 46; no. 4; pp. 429 - 439
• Main Authors: Song, Ha-Yeon, Choi, Dahye, Han, Dong-Min, Kim, Dae-Hyuk, Kim, Jung-Mi
• Format: Journal Article
• Language: English
• Published: Korea (South) Taylor & Francis 01.01.2018; Taylor & Francis Group; 한국균학회
• Subjects: color complementation; PPTase gene; Aspergillus nidulans; Promoter assay system; reporter; 생물학; color complementation; reporter; PPTase gene; Aspergillus nidulans; Promoter assay system
• ISSN: 1229-8093; 2092-9323
• DOI: 10.1080/12298093.2018.1548806

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, , which restores the normal pigmentation in , as a new reporter gene. The functional organization of serially deleted promoter regions of the gene and the gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase gene. Several promoter regions of the and genes were fused to the gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the and promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.