New insight and metrics to understand the ontogeny and succession of Lactobacillus plantarum subsp. plantarum and Lactobacillus plantarum subsp. argentoratensis
Lactobacillus plantarum is one of the most extensively studied Lactobacillus species because of its presence in a variety of environmental niches, versatility, and metabolic capabilities, resulting in the use of this organism in many industrial applications. However, although extensive effort has be...
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Published in | Scientific reports Vol. 8; no. 1; pp. 6029 - 9 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
16.04.2018
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Lactobacillus plantarum
is one of the most extensively studied
Lactobacillus
species because of its presence in a variety of environmental niches, versatility, and metabolic capabilities, resulting in the use of this organism in many industrial applications. However, although extensive effort has been invested in screening this species from a variety of habitats, a reliable and accurate method for studying the succession and ontogeny of this organism in complex ecosystems is still required to confirm the activity of
L. plantarum
at the subspecies level. Therefore, in this study, novel subspecies-specific genes for the quantitative detection of two
L. plantarum
subspecies were identified by comparative genomic analysis. The specificity of primer sets for selected genes specific to each targeted microbe was confirmed in kimchi samples. Interestingly, in all the kimchi samples at 4 °C, the presence of
L. plantarum
subsp.
argentoratensis
was not observed. Hence, we found that low temperatures markedly affected the ontogeny of
L. plantarum
subsp.
argentoratensis
during kimchi fermentation. Subsequently, this touchstone method will offer new insight and metrics to understand the ontogeny and succession of
L. plantarum
subsp.
plantarum
and
L. plantarum
subsp.
argentoratensis
in various niches. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-018-24541-6 |