An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness

• Published in: Scientific reports Vol. 8; no. 1; pp. 8981 - 13
• Main Authors: Galarza Torre, Asier, Shaw, Joshua E., Wood, Amber, Gilbert, Hamish T. J., Dobre, Oana, Genever, Paul, Brennan, Keith, Richardson, Stephen M., Swift, Joe
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.06.2018; Nature Publishing Group
• Subjects: 13/100; 14; 14/1; 14/35; 14/63; 631/1647/767; 631/532/2074; 631/57; 631/61/490; 639/166/985; 96/95; Aged; Aged, 80 and over; Atomic force microscopy; Cell Line, Transformed; Cell spreading; Cytology; Female; Gene Expression Regulation - drug effects; Gene regulation; Humanities and Social Sciences; Humans; Hydrogels; Hydrogels - chemistry; Hydrogels - pharmacology; Male; Mechanical properties; Mechanotransduction, Cellular; Mesenchymal Stem Cells - metabolism; Mesenchymal Stem Cells - ultrastructure; Mesenchyme; Microscopy, Atomic Force; Middle Aged; multidisciplinary; Proteomics; Regenerative medicine; Science; Science (multidisciplinary); Stem cells; Substrates; Tissue engineering; Transcription; Translocation; Yes-associated protein
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-27346-9

The mechanical environment can influence cell behaviour, including changes to transcriptional and proteomic regulation, morphology and, in the case of stem cells, commitment to lineage. However, current tools for characterizing substrates’ mechanical properties, such as atomic force microscopy (AFM), often do not fully recapitulate the length and time scales over which cells ‘feel’ substrates. Here, we show that an immortalised, clonal line of human mesenchymal stem cells (MSCs) maintains the responsiveness to substrate mechanics observed in primary cells, and can be used as a reporter of stiffness. MSCs were cultured on soft and stiff polyacrylamide hydrogels. In both primary and immortalised MSCs, stiffer substrates promoted increased cell spreading, expression of lamin-A/C and translocation of mechano-sensitive proteins YAP1 and MKL1 to the nucleus. Stiffness was also found to regulate transcriptional markers of lineage. A GFP-YAP/RFP-H2B reporter construct was designed and virally delivered to the immortalised MSCs for in situ detection of substrate stiffness. MSCs with stable expression of the reporter showed GFP-YAP to be colocalised with nuclear RFP-H2B on stiff substrates, enabling development of a cellular reporter of substrate stiffness. This will facilitate mechanical characterisation of new materials developed for applications in tissue engineering and regenerative medicine.