Variable frequency of polyomavirus SV40 and herpesvirus EBV in lymphomas from two different urban population groups in Houston, TX

• Published in: Journal of clinical virology Vol. 46; no. 2; pp. 154 - 160
• Main Authors: Toracchio, Sonia, Kozinetz, Claudia A, Killen, Deanna E, Sheehan, Andrea M, Banez, Eugenio I, Ittmann, Michael M, Sroller, Vojtech, Butel, Janet S
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2009; Elsevier
• Subjects: Age; Allergy and Immunology; Antigens, Polyomavirus Transforming - metabolism; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Cross-Sectional Studies; Demography; EBER; EBV; Epstein-Barr virus; Epstein-Barr Virus Infections - epidemiology; Epstein-Barr Virus Infections - virology; Female; Fundamental and applied biological sciences. Psychology; Herpesvirus; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - isolation & purification; Hospitals; Human infections; Human viral diseases; Humans; Immunohistochemistry; Infectious Disease; Infectious diseases; Lymphoma; Lymphoma - epidemiology; Lymphoma - virology; Male; Medical sciences; Microbiology; Middle Aged; Miscellaneous; Polymerase Chain Reaction; Polyomavirus; Polyomavirus Infections - epidemiology; Polyomavirus Infections - virology; Population studies; Real-time quantitative PCR; Ribonuclease P; Ribonuclease P - genetics; Simian virus 40; Simian virus 40 - genetics; Simian virus 40 - isolation & purification; Statistics, Nonparametric; SV40; T-antigen; Texas - epidemiology; Tumor Virus Infections - epidemiology; Tumor Virus Infections - virology; Tumors; Urban populations; Viral diseases; Virology; Viruses; Texas; Ben Taub General Hospital; Polyomavirus; VAMC; RQ-PCR; non-Hodgkin lymphoma; EBER; large tumor antigen; BTGH; Lymphoma; real-time quantitative PCR; NHL; Human infections; SV40; EBV; T-ag; simian virus 40; T-antigen; Epstein–Barr virus; Michael E. DeBakey Veterans Affairs Medical Center; PCR; polymerase chain reaction; Real-time quantitative PCR; Microbiology; Epstein Barr virus; Papovaviridae; Lymphoproliferative syndrome; Quantitative analysis; Gammaherpesvirinae; Human; Herpesviridae; Malignant hemopathy; Real time; Simian virus 40; Infection; Virus; Antigen; Polymerase chain reaction; Viral disease; Cancer
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2009.06.023

Abstract Background Studies have reported differing frequencies of detection of polyomavirus simian virus 40 (SV40) in association with human lymphomas. Objective We addressed the hypothesis that SV40 positivity in lymphomas can vary among sampled populations. Study design Archival paraffin-embedded lymphoma specimens ( n = 171) from patients at two urban hospitals in Houston, TX, USA, were analyzed following a cross-sectional study design. Extracted DNAs were characterized by quantitative polymerase chain reaction for the cellular RNase P gene and for SV40 and herpesvirus Epstein–Barr virus (EBV) sequences. Results Patient characteristics of the two study populations differed significantly whereas the classification of tumor types studied did not. SV40 DNA was detected more frequently in lymphomas from the public hospital population (10/44, 23%) than in lymphomas from the veterans’ hospital (VAMC) (4/127, 3%; P < 0.0001). EBV detection in lymphomas also differed between the two groups (17/44, 39% vs. 23/127, 18%; P = 0.01). SV40 positivity was associated with a younger age category of VAMC lymphoma patients ( P = 0.02). Expression of T-antigen was detected by immunohistochemistry in half of lymphomas that contained SV40 DNA. Variation was observed in the quality and quantity of DNA recovered from paraffin-embedded specimens, but there was no difference in recoveries of DNA from samples from the two hospitals. Conclusions This study demonstrated that, in a direct comparison, the prevalence of SV40 DNA in lymphomas can differ significantly between groups with different demographic distributions.