Characterization of phenolic compounds from Eugenia supra-axillaris leaf extract using HPLC-PDA-MS/MS and its antioxidant, anti-inflammatory, antipyretic and pain killing activities in vivo

• Published in: Scientific reports Vol. 9; no. 1; pp. 11122 - 12
• Main Authors: Hegazi, Nesrine M., Sobeh, Mansour, Rezq, Samar, El-Raey, Mohamed A., Dmirieh, Malak, El-Shazly, Assem M., Mahmoud, Mona F., Wink, Michael
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31.07.2019; Nature Publishing Group
• Subjects: 101/58; 631/154/1435; 631/154/433; Analgesics; Analgesics - pharmacology; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - pharmacology; Antioxidants; Antioxidants - physiology; Antipyretics - pharmacology; Chromatography, High Pressure Liquid - methods; Cyclooxygenase-1; Cyclooxygenase-2; Diclofenac; Edema; Edema - drug therapy; Edema - metabolism; Eugenia - chemistry; Eugenia axillaris; High-performance liquid chromatography; Humanities and Social Sciences; Inflammation; Inflammation - drug therapy; Leaves; Leukocyte migration; Liquid chromatography; Male; Mice; multidisciplinary; Oxidation; Oxidative stress; Oxidative Stress - drug effects; Pain; Pain - drug therapy; Pain - metabolism; Peritoneum; Phenolic compounds; Phenols; Phenols - chemistry; Plant extracts; Plant Extracts - pharmacology; Plant Leaves - chemistry; Polyphenols; Rats; Rats, Wistar; Reactive oxygen species; Reactive Oxygen Species - metabolism; Science; Science (multidisciplinary); Tandem Mass Spectrometry - methods
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-46946-7

Reactive oxygen species (ROS) are involved in the pathophysiology of several health disorders, among others inflammation. Polyphenols may modulate ROS related disorders. In this work, thirty-two phenolic compounds were tentatively identified in a leaf extract from Eugenia supra-axillaris Spring. ex Mart. using HPLC-MS/MS, five of which were also individually isolated and identified. The extract displayed a substantial in vitro antioxidant potential and was capable of decreasing ROS production and hsp-16.2 expression under oxidative stress conditions in vivo in the Caenorhabditis elegans model. Also, the extract showed higher inhibitory selectivity towards COX-2 than COX-1 in vitro with higher selectivity towards COX-2 than that of diclofenac. The extract also exhibited anti-inflammatory properties: It attenuated the edema thickness in a dose dependent fashion in carrageenan-induced hind-paw odema in rats. In addition, the extract reduced the carrageenan-induced leukocyte migration into the peritoneal cavity at the highest dose. Furthermore, the extract showed antipyretic and analgesic activities in a mouse model. Eugenia supra-axillaris appears to be a promising candidate in treating inflammation, pain and related oxidative stress diseases.