Assessment of the nutritional status of field-caught larval Pacific bluefin tuna by RNA/DNA ratio based on a starvation experiment of hatchery-reared fish

• Published in: Journal of experimental marine biology and ecology Vol. 354; no. 1; pp. 56 - 64
• Main Authors: Tanaka, Yosuke, Satoh, Keisuke, Yamada, Harumi, Takebe, Takayuki, Nikaido, Hideki, Shiozawa, Satoshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.01.2008; Elsevier Science
• Subjects: Agnatha. Pisces; Animal and plant ecology; Animal, plant and microbial ecology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Marine; Nutritional status; Pacific bluefin tuna; RNA/DNA ratio; Sea water ecosystems; Starvation; Synecology; Thunnus orientalis; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; I, Pacific; Starvation; RNA/DNA ratio; Nutritional status; Pacific bluefin tuna; Fasting; RNA; Hatchery; Marine environment; Vertebrata; Thunnus thynnus; Larva; DNA; Pisces; Ratio
• ISSN: 0022-0981; 1879-1697
• DOI: 10.1016/j.jembe.2007.10.007

RNA/DNA ratio is a useful and reliable indicator of the nutritional status of fish larvae and juveniles. In order to assess the nutritional status of field-caught larval Pacific bluefin tuna Thunnus orientalis (Temminck et Schlegel), starvation experiments of hatchery-reared larvae were conducted and changes in the RNA/DNA ratio of fed and starved larvae were analyzed. Starvation experiments were conducted every 3 days after first feeding. The survival rate of Pacific bluefin tuna larvae ranged 10–50% after 1 day of starved conditions and growth retardation was observed immediately. These results suggest that Pacific bluefin tuna larvae have a very low tolerance to starvation. The RNA/DNA ratios of fed larvae were approximately 2.0–4.0. On the other hand, the value of starved larvae significantly decreased to 1.0–3.0. The nutritional status of 3 cohorts of field-caught tuna larvae collected in the northwestern Pacific Ocean was examined based on the value of the RNA/DNA ratio of the 1 day starved larvae. 4.35–25.77% of the cohorts were regarded as the “starving condition”, which was negatively correlated to the ambient prey densities. These findings suggest that the nutritional condition of larval Pacific bluefin tuna was influenced by the ambient prey density, and starvation itself and starvation-induced predation could greatly contribute to mortality in the larval period of Pacific bluefin tuna.