Genealogical use of chloroplast DNA variation for intraspecific studies of Aegilops tauschii Coss

• Published in: Theoretical and applied genetics Vol. 111; no. 2; pp. 265 - 271
• Main Authors: Matsuoka, Y, Mori, N, Kawahara, T
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.07.2005; Berlin Springer Nature B.V
• Subjects: Aegilops tauschii; Afghanistan; biallelic polymorphism; Biological and medical sciences; chloroplast DNA; Chloroplasts; Classical genetics, quantitative genetics, hybrids; DNA, Chloroplast - genetics; Extrachromosomal DNA; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic diversity; genetic markers; genetic polymorphism; Genetic research; Genetic Variation; Genetics of eukaryotes. Biological and molecular evolution; Geography; Grasses; Intraspecific genetic variation; Iran; loci; microsatellite repeats; Microsatellite Repeats - genetics; Molecular evolution; Mutation; Mutation - genetics; nucleotide sequences; Pakistan; Phylogeny; Poaceae - genetics; Pteridophyta, spermatophyta; Sequence Analysis, DNA; Triticum aestivum; Vegetals; Iran; Afghanistan; Pakistan; Japan; Monocotyledones; Genetic variability; Gramineae; Angiospermae; Aegilops tauschii; Chloroplast DNA; Genetics; Spermatophyta; Agriculture
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-2020-x

Intraspecific patterns of chloroplast DNA variation was studied in Aegilops tauschii Coss., the D-genome progenitor of bread wheat. Nucleotide sequences of ten chloroplast microsatellite loci were analyzed for 63 accessions that cover the central part of the species distribution. As is often the case with nuclear microsatellites, those of chloroplasts of Ae. tauschii bear complex mutations. Several types of mutations other than change in the microsatellite repeat number were found, including base substitutions and length mutations in flanking regions. In total, eight mutations were present in the flanking regions of four loci. Most mutations in the flanking regions of microsatellite repeats are associated with biallelic polymorphisms. Phylogeographic analyses showed that such biallelic polymorphisms are useful to investigate intraspecific patterns of monophyletic lineage divergence. In contrast, most microsatellite repeat sites are multiallelic, variable within intraspecific lineages, and useful to compare degrees of genetic diversity between lineages. These findings show that the chloroplast genome harbors evolutionary variations informative for intraspecific studies of Ae. tauschii and can be analyzed by genealogical approaches.